論文

2026年

2025年

• Discovery and characterization of a high-affinity G-quadruplex binding peptide via mRNA display
  Naka Kudo Ida, Yoshimasa Kawaguchi, Shiroh Futaki, Miki Imanishi
  Bioorg. Med. Chem. (in press)
  doi: 10.1016/j.bmc.2025.118543
• SARS-CoV-2 inhibition through mRNA delivery using engineered extracellular vesicles displaying the spike protein
  Yuna Nakagawa, Yoshimasa Kawaguchi, Hisaaki Hirose, Takao Hashiguchi, Joseph Lee, Akitsu Hotta, Jun Kawamoto, Michihito Sasaki, Hirofumi Sawa, Shiroh Futaki
  Biomaterials (in press)
  doi: 10.1016/j.biomaterials.2025.123594
• Microcondensate-Mediated Intracellular Infusion of mRNA Across the Plasma Membrane
  Yoshimasa Kawaguchi, Ayumi Kikkawa, Seigo Kimura, Hiroshi Abe, Shiroh Futaki
  Angew. Chem. Int. Ed. 65(1):e12139 (2026)
  doi: 10.1002/anie.202512139
• Structural Control of RNA Demethylation: G-Quadruplex Proximity Suppresses ALKBH5 Activity
  Keito Obata, Kamui Tanaka, Shiroh Futaki, Miki Imanishi
  Chem. Eur. J. 31(71):e01788 (2025)
  doi: 10.1002/chem.202501788
• KCNN4 as a genomic determinant of cytosolic delivery by the attenuated cationic lytic peptide L17E
  Masashi Kuriyama, Hisaaki Hirose, Yoshimasa Kawaguchi, Junya Michibata, Masashi Maekawa, Shiroh Futaki
  Mol. Ther. 33(2), 595-614 (2025)
  DOI: 10.1016/j.ymthe.2024.12.050
• Highly sequence-specific, timing-controllable m⁶ A demethylation by modulating RNA-binding affinity of m⁶ A erasers
  Kenko Otonari, Yuri Asami, Kosuke Ogata, Yasushi Ishihama, Shiroh Futaki, Miki Imanishi
  Chem.Commun. 61(1), 69-72 (2025)
  DOI: 10.1039/D4CC04070H
• Polysaccharide-Based Coacervate Microgel Bearing Cationic Peptides That Achieve Dynamic Cell-Membrane Structure Alteration and Facile Cytosolic Infusion of IgGs
  Junya Michibata, Yoshimasa Kawaguchi, Hisaaki Hirose, Akiko Eguchi, Sayaka Deguchi, Kazuo Takayama, Wei Xu, Takuro Niidome, Yoshihiro Sasaki, Kazunari Akiyoshi, Shiroh Futaki
  Bioconjug. Chem. 35(12), 1888-1899 (2024)
  DOI: 10.1021/acs.bioconjchem.4c00344

2024年

• An approach for the intracellular delivery of IgG via enzymatic ligation with a cell-permeable attenuated cationic amphiphilic lytic peptide
  Yoshimasa Kawaguchi, Sakahiro Terada, Shiroh Futaki
  Bioorg. Med. Chem. 111, 117835 (2024)
  DOI: 10.1016/j.bmc.2024.117835
• E3MPH16: An efficient endosomolytic peptide for intracellular protein delivery
  Yoshimasa Kawaguchi, Yuki Kawamura, Hisaaki Hirose, Megumi Kiyokawa, Momo Hirate, Tsuyoshi Hirata, Yuriko Higuchi, Shiroh Futaki
  J. Control. Release 367, 877-891 (2024)
  DOI: 10.1016/j.jconrel.2024.01.067
• Liquid droplet-mediated formulation of lipid nanoparticles encapsulating immunoglobulin G for cytosolic delivery
  Yusuke Hirai, Yoshimasa Kawaguchi, Chisato Kasahara, Hisaaki Hirose, Shiroh Futaki
  Mol. Pharmaceutics 21(4), 1653-1661 (2024)
  DOI: 10.1021/acs.molpharmaceut.3c00868
• Light-controllable cell-membrane disturbance for intracellular delivery
  Wenting Huo, Koji Miki, Huiying Mu, Takashi Osawa, Harumi Yamaguma, Yuuya Kasahara, Satoshi Obika, Yoshimasa Kawaguchi, Hisaaki Hirose, Shiroh Futaki, Yusuke Miyazaki, Wataru Shinoda, Shuji Akai, Kouichi Ohe
  J. Mater. Chem. B 12(17), 4138-4147 (2024)
  DOI: 10.1039/D3TB02956E
• Functional evaluation of an electrophilic focused library to identify a covalent inhibitor against intrinsically disordered circadian clock transcription factors
  Kazuya Yamanaka, Yoshihisa Inoue, Miki Imanishi, Junko Ohkanda
  Bioorg. Med. Chem. Lett. 98, 129588 (2024)
  DOI: 10.1016/j.bmcl.2023.129588

2023年

• Xanthine Derivatives Inhibit FTO in an L-Ascorbic Acid-Dependent Manner
  Kamui Tanaka, Akiyo Suda, Motonari Uesugi, Shiroh Futaki, Miki Imanishi
  Chem. Commun. 59(72), 10809-10812 (2023)
  DOI: 10.1039/D3CC02484A
• Inkjet-Based Intracellular Delivery System that Effectively Utilizes Cell-Penetrating Peptides for Cytosolic Introduction of Biomacromolecules through the Cell Membrane
  Mika Omura, Kenta Morimoto, Yurina Araki, Hisaaki Hirose, Yoshimasa Kawaguchi, Yukiya Kitayama, Yuto Goto, Atsushi Harada, Ikuo Fujii, Tomoka Takatani-Nakase, Shiroh Futaki, Ikuhiko Nakase
  ACS Appl. Mater. Interfaces 15(41), 47855–47865 (2023)
  DOI: 10.1021/acsami.3c01650
• Structural Dissection of Epsin‐1 N‐Terminal Helical Peptide: The Role of Hydrophobic Residues in Modulating Membrane Curvature
  Motoki Nishimura, Yoshimasa Kawaguchi, Kakeru Kuroki, Yuna Nakagawa, Toshihiro Masuda, Takayuki Sakai, Kenichi Kawano, Hisaaki Hirose, Miki Imanishi, Tomoka Takatani-Nakase, Sergii Afonin, Anne S. Ulrich, Shiroh Futaki
  Chem. Eur. J. 29(29), e202300129 (2023)
  DOI: 10.1002/chem.202300129

2022年以前

• Hemopexin as a Potential Binding Partner of Arginine-rich Cell-penetrating Peptides in Serum
  Kayo Nomura, Kenichi Kawano, Yoshimasa Kawaguchi, Yuki Kawamura, Junya Michibata, Keiko Kuwata, Koji Sugiyama, Kenji Kusumoto, Shiroh Futaki
  ACS Pharmacol. Trans. Sci. 5(8), 603–615 (2022)
  DOI: 10.1021/acsptsci.2c00043
• Split Luciferase-based Estimation of Cytosolic Cargo Concentration Delivered Intracellularly via Attenuated Cationic Amphiphilic Lytic Peptides
  Syusuke Okano, Yoshimasa Kawaguchi, Kenichi Kawano, Hisaaki Hirose, Miki Imanishi, Shiroh Futaki
  Bioorg. Med. Chem. Lett. 72, 128875 (2022)
  DOI: 10.1016/j.bmcl.2022.128875
• Stearylated Macropinocytosis-inducing Peptides Facilitating the Cellular Uptake of Small Extracellular Vesicles
  Yuna Nakagawa, Jan Vincent V. Arafiles, Yoshimasa Kawaguchi, Ikuhiko Nakase, Hisaaki Hirose, Shiroh Futaki
  Bioconjug. Chem. 33(5), 869–880 (2022)
  DOI: 10.1021/acs.bioconjchem.2c00113
• Piezo1 Activation Using Yoda1 Inhibits Macropinocytosis in A431 Human Epidermoid Carcinoma Cells
  Masashi Kuriyama, Hisaaki Hirose, Toshihiro Masuda, Masachika Shudou, Jan Vincent V. Arafiles, Miki Imanishi, Masashi Maekawa, Yuji Hara, Shiroh Futaki
  Sci. Rep. 12, 6322 (2022)
  DOI: 10.1038/s41598-022-10153-8
• L17ER4: A cell-permeable attenuated cationic amphiphilic lytic peptide
  Kenta Shinga, Takahiro Iwata, Kazuya Murata, Yoko Daitoku, Junya Michibata, Jan Vincent V. Arafiles, Kentarou Sakamoto, Misao Akishiba, Tomoka Takatani-Nakase, Seiya Mizuno, Fumihiro Sugiyama, Miki Imanishi, Shiroh Futaki
  Bioorg. Med. Chem. 61, 116728 (2022)
  DOI: 10.1016/j.bmc.2022.116728
  DOI: 10.1021/acs.joc.1c02139
• Artificial Nanocage Formed via Self-assembly of β-Annulus Peptide for Delivering Biofunctional Proteins into Cell Interiors
  Kentarou Sakamoto, Hiroto Furukawa, Jan Vincent V. Arafiles, Miki Imanishi, Kazunori Matsuura, Shiroh Futaki
  Bioconjug. Chem. 33(2), 311–320 (2022)
  DOI: 10.1021/acs.bioconjchem.1c00534
• Grafting Hydrophobic Amino Acids Critical for Inhibition of Protein-Protein Interaction on a Cell-Penetrating Peptide Scaffold
  Yuki Nagano, Jan Vincent V. Arafiles, Keiko Kuwata, Yoshimasa Kawaguchi, Miki Imanishi, Hisaaki Hirose, Shiroh Futaki
  Mol. Pharm. 19(2), 558–567 (2022)
  DOI: 10.1021/acs.molpharmaceut.1c00671
• Recognition of G-quadruplex RNA by a Crucial RNA Methyltransferase Component, METTL14
  Atsuhiro Yoshida, Takanori Oyoshi, Akiyo Suda, Shiroh Futaki, Miki Imanishi
  Nucleic Acid Res. 50(1), 449–457 (2022)
  DOI: 10.1093/nar/gkab1211
• Cytosolic Protein Delivery Using pH-Responsive, Charge-Reversible Lipid Nanoparticles
  Yusuke Hirai, Hisaaki Hirose, Miki Imanishi, Tomohiro Asai, Shiroh Futaki
  Sci. Rep. 11(1), 19896 (2021)
  DOI: 10.1038/s41598-021-99180-5
• Liquid droplet formation and facile cytosolic translocation of IgG in the presence of attenuated cationic amphiphilic lytic peptides
  Takahiro Iwata, Hisaaki Hirose, Kentarou Sakamoto, Yusuke Hirai, Jan Vincent V. Arafiles, Misao Akishiba, Miki Imanishi, Shiroh Futaki
  Angew. Chem. Int. Ed. 60(36), 19804–19812 (2021)
  DOI: 10.1002/anie.202105527
• Use of Homoarginine to Obtain Attenuated Cationic Membrane Lytic Peptides
  Kentarou Sakamoto, Misao Akishiba, Takahiro Iwata, Jan Vincent V. Arafiles, Miki Imanishi, Shiroh Futaki
  Bioorg. Med. Chem. Lett. 40, 127925 (2021)
  DOI: 10.1016/j.bmcl.2021.127925
• Discovery of a Macropinocytosis-Inducing Peptide Potentiated by Medium-Mediated Intramolecular Disulfide Formation
  Jan Vincent V. Arafiles, Hisaaki Hirose, Yusuke Hirai, Masashi Kuriyama, Maxwell Mamfe Sakyiamah, Wataru Nomura, Kazuhiro Sonomura, Miki Imanishi, Akira Otaka, Hirokazu Tamamura, Shiroh Futaki
  Angew. Chem. Int. Ed. 60(21), 11928–11936 (2021)
  DOI: 10.1002/anie.202016754
• Conversion of Cationic Amphiphilic Lytic Peptides to Cell-Penetration Peptides
  Hao-Hsin Yu, Kentarou Sakamoto, Misao Akishiba, Naoki Tamemoto, Hisaaki Hirose, Ikuhiko Nakase, Miki Imanishi, Fatemeh Madani, Astrid Gräslund, Shiroh Futaki
  Pept. Sci. 112(1), e24144 (2020)
  DOI: 10.1002/pep2.24144
• Programmable RNA Methylation and Demethylation using PUF RNA Binding Proteins
  Kouki Shinoda, Akiyo Suda, Kenko Otonari, Shiroh Futaki, Miki Imanishi
  Chem. Commun. 56(9), 1365–1368 (2020)
  DOI: 10.1039/c9cc09298f
• Stimulating Macropinocytosis for Intracellular Nucleic Acid and Protein Delivery: A Combined Strategy with Membrane-Lytic Peptides To Facilitate Endosomal Escape
  Jan Vincent V. Arafiles, Hisaaki Hirose, Misao Akishiba, Shogo Tsuji, Miki Imanishi, Shiroh Futaki
  Bioconjug. Chem. 31(3), 547–553 (2020)
  DOI: 10.1021/acs.bioconjchem.0c00064
• Enhancing the Activity of Membrane Remodeling Epsin-Peptide by Trimerization
  Wei-Yuan Hsu, Toshihiro Masuda, Sergii Afonin, Takayuki Sakai, Jan Vincent V. Arafiles, Kenichi Kawano, Hisaaki Hirose, Miki Imanishi, Anne S. Ulrich, Shiroh Futaki
  Bioorg. Med. Chem. Lett. 30(12), 127190 (2020)
  DOI: 10.1016/j.bmcl.2020.127190
• Effective RNA Regulation by Combination of Multiple Programmable RNA-Binding
  Misaki Sugimoto, Akiyo Suda, Shiroh Futaki, Miki Imanishi
  Appl. Sci. 10(19), 6803 (2020)
  DOI: 10.3390/app10196803
• Identification of Synthetic Inhibitors for the DNA Binding of Intrinsically Disordered Circadian Clock Transcription Factors
  Yusuke Hosoya, Wataru Nojo, Isao Kii, Takanori Suzuki, Miki Imanishi, Junko Ohkanda
  Chem. Commun. 56(76), 11203–11206 (2020)
  DOI: 10.1039/d0cc04861e
• Dipicolylamine/Metal Complexes that Promote Direct Cell-Membrane Penetration of Octaarginine
  Yoshimasa Kawaguchi, Shoko Ise, Yusuke Azuma, Toshihide Takeuchi, Kenichi Kawano, Toan Khanh Le, Junko Ohkanda, Shiroh Futaki
  Bioconjug. Chem. 30(2), 454–460 (2019)
  DOI: 10.1021/acs.bioconjchem.8b00691
• Loosening of Lipid Packing by Cell Surface Recruitment of Amphiphilic Peptides by Coiled-coil Tethering
  Takayuki Sakai, Kenichi Kawano, Masatomo Iino, Toshihide Takeuchi, Miki Imanishi, Shiroh Futaki
  ChemBioChem 20(16), 2015–2016 (2019)
  DOI: 10.1002/cbic.201900347
• Modular Redesign of a Cationic Lytic Peptide to Promote the Endosomal Escape of Biomacromolecules
  Yusuke Azuma, Haruka Imai, Yoshimasa Kawaguchi, Ikuhiko Nakase, Hiroshi Kimura, Shiroh Futaki
  Angew. Chem. Int. Ed. 57(39), 12771–12774 (2018)
  DOI: 10.1002/anie.201807534
• Sequence-specific 5mC Detection in Live Cells Based on the TALE-split Luciferase Complementation System
  Shogo Tsuji, Kouki Shinoda, Shiroh Futaki, Miki Imanishi
  Analyst 143(16), 3793–3797 (2018)
  DOI: 10.1039/c8an00562a
• Nested PUF Proteins: Extending Target RNA Elements for Gene Regulation
  Kouki Shinoda, Shogo Tsuji, Shiroh Futaki, Miki Imanishi
  ChemBioChem (2018)
  DOI: 10.1002/cbic.201700458
• Detection of N6-methyladenosine Based on the Methyl-sensitivity of MazF RNA Endonuclease
  Miki Imanishi, Shogo Tsuji, Akiyo Suda, Shiroh Futaki
  Chem. Commun. 53(96), 12930–12933 (2017)
  DOI: 10.1039/c7cc07699a
• Cytosolic Antibody Delivery by Lipid-sensitive Endosomolytic Peptide
  Misao Akishiba, Toshihide Takeuchi, Yoshimasa Kawaguchi, Kentarou Sakamoto, Hao-Hsin Yu, Ikuhiko Nakase, Tomoka Takatani-Nakase, Fatemeh Madani, Astrid Gräslund, Shiroh Futaki
  Nat. Chem. 9(8), 751–761 (2017)
  DOI: 10.1038/NCHEM.2779

総説類

2026年

2025年

• Macropinocytosis and the Related Actin-Driven Cellular Uptake Pathways for Extracellular Fine Particles
  Siroh Futaki, Hisaaki Hirose, Yoshimasa Kawaguchi
  Extracellular Fine Particles Extracellular Fine Particles (2025)
  DOI: 10.1021/acs.bioconjchem.4c00344

2024年

• Finding ways into the cytosol: Peptide-mediated approaches for delivering proteins into cells
  Yoshimasa Kawaguchi, Shiroh Futaki
  Curr. Opin. Chem. Biol. 81, 102482 (2024)
  DOI: 10.5360/membrane.48.60

2023年以前

• Modified Nucleobase-Specific Gene Regulation Using Engineered Transcription Activator-Like Effectors
  Shogo Tsuji, Miki Imanishi
  Adv. Drug Deliv. Rev., 147, 59-65 (2019)
  DOI: 10.1016/j.addr.2019.08.011
• Cellular Uptake of Arginine-Rich Cell-Penetrating Peptides and the Contribution of Membrane-Associated Proteoglycans
  Ikuhiko Nakase, Yoshimasa Kawaguchi, Motoyoshi Nomizu, Shiroh Futaki
  Trends Glycosci. Glycotech., 27(155), 81-88 (2015)
• New functions of zinc fingers revealed by substitution of conserved residues.
  Imanishi M, Sugiura Y.
  Zinc Fingers: Structure, Properties and Applications (Nova Science Publishers, Inc.), 141-154 (2012)
• Design of artificial DNA binding proteins toward control and elucidation of cellular functions.
  Imanishi M.
  Yakugaku Zasshi
• ゲノムを標的にした人工ジンクフィンガータンパク質の創製
  今西未来
  ファルマシア Vol.48, No. 3, 201-205 (2012)
• マルチ亜鉛フィンガー型人工転写因子の創製と機能
  (Artificial Transcription Factors Based on Multi-Zinc Finger Motifs)
  森崎達也, 今西未来, 二木史朗, 杉浦幸雄
  薬学雑誌, 130(1), 45-48 (2010)
• New Redesigned Zinc-Finger Proteins: Design Strategy and Its Application
  Shigeru Negi, Miki Imanishi, Makoto Matsumoto, Yukio Sugiura
  Chemistry, 14(11), 3236-3249 (2008)
  PMID: 18236477
• 遺伝子を標的とした人工亜鉛フィンガータンパク質の創製
  今西未来
  化学工業, 57(9), 667-671 (2006)